Biosynthesis of Copper Oxide Nanoparticles Using Streptomyces MHM38 and Its Biological Applications

Biosynthesis methods employing microorganisms have emerged as an eco-friendly, clean, and viable alternative to chemical physical processes. The present study reports the synthesis of copper oxide nanoparticles (CuONPs) using cell-free culture supernatant marine Streptomyces sp. MHM38. For optimized production CuONPs, influence some parameters, such concentration sulfate (CuSO4), reaction time, filtrate substrate ratio, pH, was studied. 5 mM CuSO4 optimal for nanoparticle (NP) production. Well-defined CuONP formation occurred after 60 min incubation when equal volume (cell-free supernatant) (CuSO4 solution) added. UV-visible spectroscopy analysis CuONPs exhibited a peak at 550 nm, which corresponds surface plasmon resonance CuONPs. Most particles were spherical 1.72–13.49 nm measured transmission electron microscope. antimicrobial activity determined well diffusion method against Enterococcus faecalis ATCC 29212, Salmonella typhimurium 14028, Pseudomonas aeruginosa 9027, Escherichia coli 8939, fungi (Rhizoctonia solani, Fusarium Aspergillus niger), yeast (Candida albicans 10237). highest activities recorded Candida 10237, whereas 14028 8939 showed less activity. biochemical findings groups significant ( p < 0.05 ) with diminished levels alanine aminotransferase (ALT), aspartate (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total direct bilirubin, urea, creatinine compared paracetamol group. Nonenzymatic enzymatic antioxidants significantly elevated id="M2"> in SOD GSH levels, exceptionally low nitric (NO) malondialdehyde (MAD) found histopathological examination assured impact improving paracetamol-induced liver damage.